Tissue Bio Lab

Hi all! And welcome to histology! Hope you all are ready to hit the ground running as we start a new semester. For most of you, histology is new territory and may seem a little intimidating at first, but I promise this class isn't difficult as long as you have the right tools and spend a little time familiarizing yourself with the world of tissues. 

Some tips for doing well in this class:

1. Use time in lab wisely - maximize the time you spend in lab; the more memorizing and identifying you do during class, the less cramming you'll have to do come quiz time.

2. Don't get discouraged - if you miss a couple points on the first few quizzes, don't despair! Remember, not only will your lowest quiz score be dropped but the final is worth half of your grade - identify your mistakes early so you don't repeat them on the final!

3. Look for landmarks - it's easy to get lost when looking at cells up close - look for distinct features common to all tissues of the same type - we will try to point out as many of these landmarks as possible in lab to make identification easier.

4. Look at as many different kinds of slides as you can - I can't stress this one enough - just as in anatomy where no two set of forearm muscles looked exactly the same, so no two preparations of even the exact same tissue will look exactly the same. Use Google images as well as the links to the right to access the University of Michigan's and others' slide banks - looking at as many slides as possible will make a very real difference in your success in this class!

This week in lab we are studying the structures of Development. Below is a list of the key terms and concepts you should be familiar with for next weeks' quiz. I'm excited to work with you as we discover the beauty and simplicity of our bodies' tissues. Good luck, and welcome to histology!!!!

Development: Terms and Concepts

Key: Know the anatomical and histological names for the following bolded structures; assume that you will be required to find the structures indicated by * on your own slides.

***This list is not guaranteed to be exhaustive, and only includes terms from this unit. While we will not focus on quiz information from previous weeks, knowledge of previous material may be useful***

Cytology

Tissue Preparation

Be able to differentiate between the types of tissue section

·    Transverse

·    Longitudinal

Stains

Know the following stains and what they are used for

·    Hemotoxylin & Eosin

·    Diff-Quick or Wright Stain

·    Orcein-Resorcein or Verhoeffs

·    Silver Stain or Argentaffin

·    Supravital or Brilliant Cresyl Blue

Developmental Biology

Fertilization

Understand the process of fertilization including the following reactions

·    Acrosomal Reaction

·    Cortical Reaction

Cleavage

Understand the process of cleavage and be able to recognize or differentiate among the following terms

·    Blastomere

·    Morula

·    Blastula

Gastrulation

Understand the process of gastrulation, know the three primary germ layers, and the types of tissues the germ layers give rise to

·    Ectoderm

·    Mesoderm

·    Endoderm

Neurulation

Understand neurulation to the extent outlined in the lab manual

Embryonic Chick

Recognize the following embryonic structures and know what they differentiate into.

·    Ectoderm*

·    Mesoderm*

·    Chordamesoderm (notochord)*

·    Dermamyotome*

·    Sclerotome*

·    Splanchnic mesoderm*

·    Somatic Mesoderm*

·    Intermediate Mesoderm*

·    Endoderm*

·    Neural Tube*

·    Dorsal Aorta*

·    Gut Endoderm*

Neural Crest Cells

Understand neural crest cells to the extent outlined in your lab manual.